Effects of C-Terminal Truncation on Autocatalytic Processing of Bacillus licheniformis gamma-Glutamyl Transpeptidase

The role of the C-terminal region of Bacillus licheniformis gamma-glutamyl transpeptidase (BlGGT) was investigated by deletion analysis. Seven C-terminally truncated BlGGTs lacking 581-585, 577-585, 576-585, 566-585, 558-585, 523-585, and 479-585 amino acids, respectively, were generated by site-directed mutagenesis. Deletion of the last nine amino acids had no appreciable effect on the autocatalytic processing of the enzyme, and the engineered protein was active towards the synthetic substrate L-gamma-glutamyl-p-nitroanilide. However, a further deletion to Val576 impaired the autocatalytic processing. In vitro maturation experiments showed that the truncated BlGGT precursors, pro-Delta (576-585), pro-Delta (566-585), and pro-Delta(558-585), could partially precede a time-dependent autocatalytic process to generate the L- and S-subunits, and these proteins showed a dramatic decrease in catalytic activity with respect to the wild-type enzyme. The parental enzyme (BlGGT-4aa) and BlGGT were unfolded biphasically by guanidine hydrochloride (GdnCl), but Delta(577-585), Delta(576-585), Delta(566-585), Delta(558-585), Delta(523-585), and Delta(479-585) followed a monophasic unfolding process and showed a sequential reduction in the GdnCl concentration corresponding to half effect and. Delta G(0) for the unfolding. BlGGT-4aa and BlGGT sedimented at similar to 4.85 S and had a heterodimeric structure of approximately 65.23 kDa in solution, and this structure was conserved in all of the truncated proteins. The frictional ratio (f/f(o)) of BlGGT-4aa, BlGGT, Delta(581-585), and Delta(577-585) was 1.58, 1.57, 1.46, and 1.39, respectively, whereas the remaining enzymes existed exclusively as precursor form with a ratio of less than 1.18. Taken together, these results provide direct evidence for the functional role of the C-terminal region in the autocatalytic processing of BlGGT

An Architecture of IoT Service Delegation and Resource Allocation Based on Collaboration between Fog and Cloud Computing

Despite the wide utilization of cloud computing (e.g., services, applications, and resources), some of the services, applications, and smart devices are not able to fully benefit from this attractive cloud computing paradigm due to the following issues: (1) smart devices might be lacking in their capacity (e.g., processing, memory, storage, battery, and resource allocation), (2) they might be lacking in their network resources, and (3) the high network latency to centralized server in cloud might not be efficient for delay-sensitive application, services, and resource allocations requests. Fog computing is promising paradigm that can extend cloud resources to edge of network, solving the abovementioned issue. As a result, in this work, we propose an architecture of IoT service delegation and resource allocation based on collaboration between fog and cloud computing. We provide new algorithm that is decision rules of linearized decision tree based on three conditions (services size, completion time, and VMs capacity) for managing and delegating user request in order to balance workload. Moreover, we propose algorithm to allocate resources to meet service level agreement (SLA) and quality of services (QoS) as well as optimizing big data distribution in fog and cloud computing. Our simulation result shows that our proposed approach can efficiently balance workload, improve resource allocation efficiently, optimize big data distribution, and show better performance than other existing methods

Field Building Leadership Initiative: Advancing Ecohealth in Southeast Asia - The first year journey

The Ecosystem approach to health, or Ecohealth is a useful tool to address health and environmental issues in developing countries, particularly in South East Asia (SEA), where agricultural intensification processes have profound implications for ecosystems and health. The Field Building Leadership Initiative (FBLI) Advancing Ecohealth in SEA program aims to build the field of Ecohealth by integrating research, training, policy and networking to focus on solving human health problems associated with agricultural intensification in SEA countries. This paper aims at updating the one-year journey of the program from October 2011 until October 2012 and providing insights into the plan for the coming years. A number of activities of the project’s research component, capacity building, and knowledge translation have been completed in line with the proposal. Outcomes and outputs of the one-year implementation are also presented

Sosekima : proceedings of socio-economics, innovation and management of the Java sea pelagic fisheries

In 1994, the Mekong Delta had 14,337 fishing boats. They exploited 404,500 tons of products from 220,000 km2 of the Economic Excluse Zone, that covers respectively 44% and 60% of national and regional fisheries' production. Traditionally developed since the beginning of the twentieth century, the industry has undergone different stages by political changes. In recent years, dramatic changes arose from the motorisation and the introduction of high productive new fishing gears (trawl net, gill net and encircling net) for off-shore fishing to prevent the exploitation in the coastal areas. Meanwhile, longlines, stow nets, scrape gears are also important. The private sector covers a large proportion in the total production output. Cost structure and profitability depend on fishing types, scales and sectors. The study also presents recommendtions for further development of the industry. Particular emphasis is placed on gaining knowledge of resource management and fishing technology. (Résumé d'auteur

Fatal poisoning by butachlor and chlornitrofen ingested from a bottle marked as nitrofen

Nitrofen has been banned in Taiwan since January 1, 1983 due to its tetrogenicity. A 78 y-o female consumed about 500 ml of herbicide. labeled as nitrofen, in a suicide attempt. Consciousness disturbance occurred immediately. After 9 h, bloody-tarry stool was noted. Hematemesis occurred 19 h later. Hepatotoxicity and nephrotoxicity also developed. Twitching of head and both upper extremities lasting 20-30 sec and up to 5 min developed on day 3. Sometimes an upward gaze with the face turned to the right or a convulsion of the right upper extremity was observed. EEG showed alpha coma, generalized slow spike-and-wave complexes, and isolated generalized sharp waves mainly in the left occipital area. On day 7, endotracheal intubation was performed due to apnea: she expired on day 9. The pesticide was found to contain butachlor and chlornitrofen. The Agricultural Committee prohibited the marketing of chlornitrofen due to its generation of turners in animals. The combination of butachlor and chlornitrofen can result in consciousness disturbance, leucocytosis, gastrointestinal hemorrhage, rhabdomyolysis, hypocalcemia, hypoalbuminemia, elevated amylase, nephrotoxicity, hepatotoxicity, seizures and death. The cytotoxicity of butachlor may be related to the patient's death. Active components of pesticides should be checked if the clinical course era poisoned patient is unusual to allow appropriate interventions

The three-dimensional groundwater salinity distribution and fresh groundwater volumes in the Mekong Delta, Vietnam, inferred from geostatistical analyses

Over the last decades, economic developments in the Vietnamese Mekong Delta have led to a sharp increase in groundwater pumping for domestic, agricultural and industrial use. This has resulted in alarming rates of land subsidence and groundwater salinization. Effective groundwater management, including strategies to work towards sustainable groundwater use, requires knowledge about the current groundwater salinity distribution, in particular the available volumes of fresh groundwater. At the moment, no comprehensive dataset of the spatial distribution of fresh groundwater is available. To create a 3D model of total dissolved solids (TDS), an existing geological model of the spatial distribution and thickness of the aquifers and aquitards is updated. Next, maps of drainable porosity for each aquifer are interpolated based on the sedimentological description of the borehole data. Measured TDS in groundwater, inferred TDS from resistivity measurements in boreholes and soft incomplete data (derived from measurements in boreholes and data from domestic wells) are combined in an indicator kriging routine to obtain the full probability distribution of TDS for each (x,y,z) location. This statistical distribution of TDS combined with drainable porosity yields estimates of the volume of fresh groundwater (TDSĝ€¯<ĝ€¯1ĝ€¯gĝ€¯L-1) in each aquifer. Uncertainty estimates of these volumes follow from a Monte Carlo analysis (sequential indicator simulation). Results yield an estimated fresh groundwater volume for the Mekong Delta of 867 billion cubic metres with an uncertainty range of 830-900 billion cubic metres, which is somewhat higher than previous assessments of fresh groundwater volumes. The resulting dataset can for instance be used in groundwater flow and salt transport modelling as well as aquifer storage and recovery projects to support informed groundwater management decisions, e.g. to prevent further salinization of the Mekong Delta groundwater system and land subsidence, and is available at 10.5281/zenodo.4441776 (Gunnink et al., 2021)